UV-Vis Tutorial | Part 1: Intro to Measuring Nanoparticles

Demonstration of how to accurately measure the optical spectra of solutions of nanoparticles using a UV-Vis (UV-Visible) spectrophotometer. In this demo, we use an Agilent 8453 spectrophotometer to measure a sample of nanoComposix‘s NanoXact 10 nm silver nanospheres, but the same techniques can be applied to many different instruments and sample types.

Video discusses:

• Background of UV-Vis spectrophotometry
• Blanking the instrument
• Measuring your sample
• Other tips & things to watch out for when measuring UV-Vis

Guidelines for UV-Vis Analysis handbook:

Other useful links:

• Optical Properties of Silver Nanoparticles:
• Optical Properties of Gold Nanoparticles:
• Introduction to Plasmonic Nanomaterials:
• Overview of Nanoparticle Characterization Techniques:

Part 2 of this series can be found here:

Let us measure your sample for you! Find out more about our UV-Vis measurement services and other nanoparticle characterization services at

Thanks for watching! Please comment below with feedback and suggestions of what else you’d like to see from us!

nanoComposix Team
(858) 565-4227


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